Laurdan Monitors Different Lipids Content in Eukaryotic Membrane During Embryonic Neural Development
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چکیده
منابع مشابه
Ultrastructural changes in the avian vitelline membrane during embryonic development.
The vitelline (yolk) membrane of the avian egg plays a dual role during early embryonic development; it encloses the yolk and provides a substratum for expansion of the embryo (Fig. 1). Expansion appears to be dependent upon the movement of cells at the edge of the blastoderm which is intimately associated with the inner layer of the vitelline membrane (New, 1959; Bellairs, 1963). The blastoder...
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The electrophysiological properties of embryonic chick hearts (ventricles) change during development; the largest changes occur between days 2 and 8. Resting potential (E(m)) and peak overshoot potential (+E(max)) increase, respectively, from -35 mv and +11 mv at day 2 to -70 mv and +28 mv at days 12-21. Action potential duration does not change significantly. Maximum rate of rise of the action...
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The physical properties and chemical composition of microsomal membranes were examined during a 7 day period of ice encasement in crown tissue of winter wheat (Triicum aestivum L. cv Norstar). Membrane damage, detected as an increase in microviscosity and electrolyte leakage, began between 1 and 3 days of icing, and was associated with a reduction in the recovery of microsomal membranes from st...
متن کاملUse of Laurdan fluorescence in studying plasma membrane organization of polymorphonuclear leukocytes during the respiratory burst.
The changes in plasma membrane polarity of polymorphonuclear leukocytes (PMN) during the activation of the respiratory burst were investigated by measuring the steady-state fluorescence emission spectra of 2-dimethylamino(6-lauroyl) naphthalene (Laurdan), which is known to be incorporated at the hydrophobic-hydrophilic interface of the bilayer, displaying spectral sensitivity to the polarity of...
متن کاملLaurdan and Di-4-ANEPPDHQ probe different properties of the membrane
Lipid packing is a crucial feature of cellular membranes. Quantitative analysis of membrane lipid packing can be achieved using polarity sensitive probes whose emission spectrum depends on the lipid packing. However, detailed insights into the exact mechanisms that cause the changes in the spectra are necessary to interpret experimental fluorescence emission data correctly. Here, we analysed fr...
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ژورنال
عنوان ژورنال: Cell Biochemistry and Biophysics
سال: 2014
ISSN: 1085-9195,1559-0283
DOI: 10.1007/s12013-014-9982-8